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NADPH oxidase and reactive oxygen species as signaling molecules in carcinogenesis

Gang WANG

《医学前沿（英文）》 2009年 第3卷 第1期   页码 1-7 doi: 10.1007/s11684-009-0018-5

摘要： Reactive oxygen species (ROS) are small molecule metabolites of oxygen that are prone to participate in redox reactions their high reactivity. Intracellular ROS could be generated in reduced nicotinamide-adenine dinucleotidephosphate (NADPH) oxidase-dependent and/or NADPH oxidase-independent manners. Physiologically, ROS are involved in many signaling cascades that contribute to normal processes. One classical example is that ROS derived from the NADPH oxidase and released in neurotrophils are able to digest invading bacteria. Excessive ROS, however, contribute to pathogenesis of various human diseases including cancer, aging, dimentia and hypertension. As signaling messengers, ROS are able to oxidize many targets such as DNA, proteins and lipids, which may be linked with tumor growth, invasion or metastasis. The present review summarizes recent advances in our comprehensive understanding of ROS-linked signaling pathways in regulation of tumor growth, invasion and metastasis, and focuses on the role of the NADPH oxidase-derived ROS in cancer pathogenesis.

关键词： free radicals     tumor     phox     cell proliferation     cancer therapy    

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Reactive oxygen species generation is essential for cisplatin-induced accelerated senescence in hepatocellular

null

《医学前沿（英文）》 2014年 第8卷 第2期   页码 227-235 doi: 10.1007/s11684-014-0327-1

摘要：

Accelerated senescence is important because this process is involved in tumor suppression and has been induced by many chemotherapeutic agents. The platinum-based chemotherapeutic agent cisplatin displays a wide range of antitumor activities. However, the molecular mechanism of cisplatin-induced accelerated senescence in hepatocellular carcinoma (HCC) remains unclear. In the present study, the growth inhibitory effect of cisplatin on HepG2 and SMMC-7721 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cellular senescence was then assessed by β-galactosidase assay. Senescence-related factors, including p53, p21, and p16, were evaluated by quantitative reverse transcription-polymerase chain reaction. Reactive oxygen species (ROS) was analyzed by flow cytometry. Our results revealed that cisplatin reduced the proliferation of HepG2 and SMMC-7721 cells in a dose- and time-dependent manner. Senescent phenotype observed in cisplatin-treated hepatoma cells was dependent on p53 and p21 activation but not on p16 activation. Furthermore, cisplatin-induced accelerated senescence depended on intracellular ROS generation. The ROS scavenger N-acetyl-L-cysteine also significantly suppressed the cisplatin-induced senescence of HepG2 and SMMC-7721 cells. In conclusion, our results revealed a functional link between intracellular ROS generation and cisplatin-induced accelerated senescence, and this link may be used as a potential target of HCC.

关键词： reactive oxygen species     senescence     cisplatin     hepatocellular carcinoma    

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Resveratrol reduces intracellular reactive oxygen species levels by inducing autophagy through the AMPK-mTOR

Jun Song, Yeping Huang, Wenjian Zheng, Jing Yan, Min Cheng, Ruxing Zhao, Li Chen, Cheng Hu, Weiping Jia

《医学前沿（英文）》 2018年 第12卷 第6期   页码 697-706 doi: 10.1007/s11684-018-0655-7

摘要：

Oxidative stress induced by free fatty acid aggravates endothelial injury, which leads to diabetic cardiovascular complications. Reduction of intracellular oxidative stress may attenuate these pathogenic processes. The dietary polyphenol resveratrol reportedly exerts potential protective effects against endothelial injury. This study determined whether resveratrol can reduce the palmitic acid (PA)-induced generation of reactive oxygen species (ROS) and further explored the underlying molecular mechanisms. We found that resveratrol significantly reduced the PA-induced endothelial ROS levels in human aortic endothelial cells. Resveratrol also induced endothelial cell autophagy, which mediated the effect of resveratrol on ROS reduction. Resveratrol stimulated autophagy via the AMP-activated protein kinase (AMPK)-mTOR pathway. Taken together, these data suggest that resveratrol prevents PA-induced intracellular ROS by autophagy regulation via the AMPK-mTOR pathway. Thus, the induction of autophagy by resveratrol may provide a novel therapeutic candidate for cardioprotection in metabolic syndrome.

关键词： resveratrol     reactive oxygen species     AMPK     mTOR     autophagy    

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concentrations of ionic silver promote the proliferation of human keratinocytes by inducing the production of reactiveoxygen species

null

《医学前沿（英文）》 2018年 第12卷 第3期   页码 289-300 doi: 10.1007/s11684-017-0550-7

摘要：

Silver-containing preparations are widely used in the management of skin wounds, but the effects of silver ions on skin wound healing remain poorly understood. This study investigated the effects of silver ions (Ag+) on the proliferation of human skin keratinocytes (HaCaT) and the production of intracellular reactive oxygen species (ROS). After treating HaCaT cells with Ag+and/or the active oxygen scavenger N-acetyl cysteine (NAC), cell proliferation and intracellular ROS generation were assessed using CCK-8 reagent and DCFH-DA fluorescent probe, respectively. In addition, 5-bromo-2-deoxyUridine (BrdU) incorporation assays, cell cycle flow cytometry, and proliferating cell nuclear antigen (PCNA) immunocytochemistry were conducted to further evaluate the effects of sub-cytotoxic Ag+ concentrations on HaCaT cells. The proliferation of HaCaT cells was promoted in the presence of 10−6 and 10−5 mol/L Ag+ at 24, 48, and 72 h. Intracellular ROS generation also significantly increased for 5–60 min after exposure to Ag+. The number of BrdU-positive cells and the presence of PCNA in HaCaT cells increased 48 h after the addition of 10−6 and 10−5 mol/L Ag+, with 10−5 mol/L Ag+ markedly increasing the cell proliferation index. These effects of sub-cytotoxic Ag+ concentrations were repressed by 5 mmol/L NAC. Our results suggest that sub-cytotoxic Ag+ concentrations promote the proliferation of human keratinocytes and might be associated with a moderate increase in intracellular ROS levels. This study provides important experimental evidence for developing novel silver-based wound agents or dressings with few or no cytotoxicity.

关键词： ionic silver     human keratinocyte     cell proliferation     reactive oxygen species     active oxygen scavenger     NAC    

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Relationship between reactive oxygen species and sodium-selenite-induced DNA damage in HepG2 cells

ZOU Yunfeng, NIU Piye, GONG Zhiyong, YANG Jin, YUAN Jing, WU Tangchun, CHEN Xuemin

《医学前沿（英文）》 2007年 第1卷 第3期   页码 327-332 doi: 10.1007/s11684-007-0063-x

摘要： Selenium compounds, as an effective chemopreventive agent, can induce apoptosis in tumor cells. Reactive oxygen species (ROS) are important mediators in apoptosis induced by various stimuli, which include chemopreventive agents. In this study, we investigated the relationship between ROS and the levels of DNA damage induced by selenite in HepG2 cells. After HepG2 cells were treated with selenite, there was a dose-dependent decrease in cell viability. The levels of ROS induced by selenite were measured by 2′, 7′-dichlorofluorescein diacetate (DCFH-DA) fluorescence, which shows a dose- and time-dependent increase in HepG2 cells. The levels of DNA damage in HepG2 increased in all cells treated with an increasing dose of selenite at 0, 2.5, 5, 10, and 20 μmol/L. N-acetylcysteine (NAC), a known antioxidant, increased cell viability and decreased ROS generation. Moreover, NAC effectively blocked DNA damage induced by selenite. These results revealed that ROS might play an important role in selenite-induced DNA damage that can be reduced by NAC treatment.

关键词： NAC     N-acetylcysteine     DNA     fluorescence     relationship    

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Association of gene variants with juvenile amyotrophic lateral sclerosis

《医学前沿（英文）》 doi: 10.1007/s11684-023-1005-y

摘要： Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive degeneration of motor neurons, and it demonstrates high clinical heterogeneity and complex genetic architecture. A variation within TRMT2B (c.1356G>T; p.K452N) was identified to be associated with ALS in a family comprising two patients with juvenile ALS (JALS). Two missense variations and one splicing variation were identified in 10 patients with ALS in a cohort with 910 patients with ALS, and three more variants were identified in a public ALS database including 3317 patients with ALS. A decreased number of mitochondria, swollen mitochondria, lower expression of ND1, decreased mitochondrial complex I activities, lower mitochondrial aerobic respiration, and a high level of ROS were observed functionally in patient-originated lymphoblastoid cell lines and TRMT2B interfering HEK293 cells. Further, TRMT2B variations overexpression cells also displayed decreased ND1. In conclusion, a novel JALS-associated gene called TRMT2B was identified, thus broadening the clinical and genetic spectrum of ALS.

关键词： TRMT2B     amyotrophic lateral sclerosis     mitochondrial complex I     tRNA methylation     reactive oxygen species    

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Visible light induces bacteria to produce superoxide for manganese oxidation

《环境科学与工程前沿（英文）》 2023年 第17卷 第2期 doi: 10.1007/s11783-023-1619-y

摘要：

● Term of manganese-oxidizing microorganisms should be reconsidered.

关键词： Mn(II) oxidation     Manganese-oxidizing bacteria     Reactive oxygen species     Mn(III/IV) oxides    

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INTERFERENCE BY NON-HOST PLANT ROOTS AND ROOT EXUDATES IN THE INFECTION PROCESSES OF PHYTOPHTHORA NICOTIANAE

《农业科学与工程前沿（英文）》 2021年 第8卷 第3期   页码 447-459 doi: 10.15302/J-FASE-2021399

摘要：

Crop rotations are widely used because they can significantly reduce the incidence of pests and diseases. The interactions between non-host roots and pathogens may be key in the inhibition of soilborne pathogens in crop rotations. Interactions between fennel (Foeniculum vulgare) roots/root exudates and Phytophthora nicotianae were investigated because of the known allelopathy between fennel and tobacco (Nicotiana tabacum). The effects of the key compounds in the fennel rhizosphere on the mycelial growth and zoospore behavior of P. nicotianae were assessed. The roots of fennel attracted P. nicotianae zoospores and inhibited their motility and the germination of cystospores, with some cystospores rupturing. 4-ethylacetophenone, vanillin and N-formylpiperidine were consistently identified in the fennel rhizosphere and were found to interfere with the infection of P. nicotianae, especially vanillin. Hyphae treated with these compounds produced more abnormal branches and accumulated reactive oxygen species. These interspecific interactions between non-host roots and pathogens were found to be an important factor in the inhibition by fennel of infection by P. nicotianae.

 

关键词： fennel and tobacco rotation     infection behavior     Phytophthora nicotianae     reactive oxygen species     vanillin    

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Biotoxicity evaluation of zinc oxide nanoparticles on bacterial performance of activated sludge at COD, nitrogen, and phosphorus reduction

《环境科学与工程前沿（英文）》 2022年 第16卷 第2期 doi: 10.1007/s11783-021-1453-z

摘要：

• ZnO-NP disrupted metabolic/catabolic balance of bacteria by affecting DHA activity.

关键词： Granular sludge     Biotoxicity     Reactive oxygen species     Extracellular polymeric substances    

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Discovery of novel ursolic acid derivatives as effective antimicrobial agents through a ROS-mediated apoptosis mechanism

《化学科学与工程前沿（英文）》 2023年 第17卷 第12期   页码 2101-2113 doi: 10.1007/s11705-023-2361-5

摘要： In response to the reduction of food production and economic losses caused by plant bacterial diseases, it is necessary to develop new, efficient, and green pesticides. Natural products are rich and sustainable source for the development of new pesticides due to their low toxicity, easy degradation, and eco-friendliness. In this study, we prepared three series of ursolic acid derivatives and assessed their antibacterial ability. Most target compounds exhibited outstanding antibacterial activities. Among them, the relative optimal EC50 values of Xanthomonas oryzae pv. oryzae and Xanthomonas axonopodis pv. citri were 2.23 (A17) and 1.39 (A16) μg·mL–1, respectively. The antimicrobial mechanism showed that compound A17 induced an excessive accumulation and production of reactive oxygen species in bacteria and damaged the cell membrane integrity to kill bacteria. More interestingly, the addition of low concentrations of exogenous hydrogen peroxide enhanced the antibacterial efficacy of compound A17 against Xanthomonas oryzae pv. oryzae. These entertaining results suggested that compound A17 induced an apparent apoptotic behavior in the tested bacteria. Overall, we developed the promising antimicrobial agents that destroyed the redox system of phytopathogenic bacteria, further demonstrating the unprecedented potential of ursolic acid for agricultural applications.

关键词： ursolic acid     antibacterial activities     reactive oxygen species     apoptosis    

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Elimination of antibiotic resistance genes and control of horizontal transfer risk by UV-based treatment of drinking water: A mini review

Virender K. Sharma, Xin Yu, Thomas J. McDonald, Chetan Jinadatha, Dionysios D. Dionysiou, Mingbao Feng

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1122-7

摘要：

Antibiotic-resistant bacteria and antibiotic resistance genes are in water bodies.

UV/chlorination method is better to remove ARGs than UV or chlorination alone.

Research on UV/hydrogen peroxide to eliminate ARGs is forthcoming.

UV-based photocatalytic processes are effective to degrade ARGs.

关键词： Antibiotic resistance bacteria     Advanced oxidation processes     Disinfection     Reactive chlorine species     Sulfate radicals     Reactive oxygen species    

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Cooperative effect between copper species and oxygen vacancy in CeZrCuO catalysts for carbon monoxide

《化学科学与工程前沿（英文）》 2021年 第15卷 第6期   页码 1524-1536 doi: 10.1007/s11705-021-2106-2

摘要： The effects of Zr doping on the existence of Cu and the catalytic performance of Ce0.7−xZrxCu0.3O2 for CO oxidation were investigated. The characterization results showed that all samples have a cubic structure, and a small amount of Zr doping facilitates Cu2+ ions entering the CeO2 lattice, but excessive Zr doping leads to the formation of surface CuO crystals again. Thus, the number of oxygen vacancies caused by the Cu2+ entering the lattice (e.g., Cu2+–□–Ce4+; □: oxygen vacancy), and the amount of reducible copper species caused by CuO crystals, varies with the Zr doping. Catalytic CO oxidation tests indicated that the oxygen vacancy and the reducible copper species were the adsorption and activation sites of O2 and CO, respectively, and the cooperative effects between them accounted for the high CO oxidation activity. Thus, the samples x = 0.1 and 0.3, which possessed the most oxygen vacancy or reducible copper species, showed the best activity for CO oxidation, with full CO conversion obtained at 110 °C. The catalyst is also stable and has good resistance to water during the reaction.

关键词： Ce–Zr–Cu–O     CO oxidation     reducible copper species     oxygen vacancy     cooperative effect    

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Review on design and evaluation of environmental photocatalysts

Xin Li, Jun Xie, Chuanjia Jiang, Jiaguo Yu, Pengyi Zhang

《环境科学与工程前沿（英文）》 2018年 第12卷 第5期 doi: 10.1007/s11783-018-1076-1

摘要：

Fundamentals on the photocatalytic degradation were systematically summarized.

Charge carrier dynamics for the photocatalytic degradation were reviewed.

Adsorption and photodegradation kinetics of reactants were highlighted.

The mechanism aspects, including O2 reduction, reactive oxidation species and key intermediates were also addressed.

Selectivity and stability of semiconductors for photodegradation were clarified.

关键词： Photocatalytic degradation     Environmental remediation     Charge carrier dynamics     Reactive oxygen species     O2 reduction    

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Light-induced variation in environmentally persistent free radicals and the generation of reactive radicalspecies in humic substances

Yafang Shi, Yunchao Dai, Ziwen Liu, Xiaofeng Nie, Song Zhao, Chi Zhang, Hanzhong Jia

《环境科学与工程前沿（英文）》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1285-2

摘要： Abstract • Light irradiation increased the concentration of free radicals on HS. • The increased spin densities on HS readily returned back to the original value. • The “unstable” free radicals induced the formation of reactive radical species. • Reactive radicals’ concentration correlated strongly with EPFRs’ concentration. Environmentally persistent free radicals (EPFRs) in humic substances play an essential role in soil geochemical processes. Light is known to induce EPFRs formation for dissolved organic matter in aquatic environments; however, the impacts of light irradiation on the variation of EPFRs in soil humic substances remain unclear. In this study, humic acid, fulvic acid, and humin were extracted from peat soil and then in situ irradiated using simulated sunlight. Electron paramagnetic resonance spectroscopy results showed that with the increasing irradiation time, the spin densities and g-factors of humic substances rapidly increased during the initial 20 min and then gradually reached a plateau. After irradiation for 2h, the maximum spin density levels were up to 1.63 × 1017, 2.06 × 1017, and 1.77 × 1017 spins/g for the humic acid, fulvic acid, and humin, respectively. And the superoxide radicals increased to 1.05 × 1014–1.46 × 1014 spins/g while the alkyl radicals increased to 0.47 × 1014–1.76 × 1014 spins/g. The light-induced EPFRs were relatively unstable and readily returned back to their original state under dark and oxic conditions. Significant positive correlations were observed between the concentrations of EPFRs and reactive radical species (R2 = 0.65–0.98, p<0.05), which suggested that the newly produced EPFRs contributed to the formation of reactive radical species. Our findings indicate that under the irradiation humic substances are likely to be more toxic and reactive in soil due to the formation of EPFRs.

关键词： Peat     Humic substances     Environmentally persistent free radicals     Light irradiation     Reactive radical species    

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Activity and characteristics of “Oxygen-enriched” highly reactive absorbent for simultaneous flue gas

Yi ZHAO,Tianxiang GUO,Zili ZANG

《环境科学与工程前沿（英文）》 2015年 第9卷 第2期   页码 222-229 doi: 10.1007/s11783-014-0636-2

摘要： An “Oxygen-enriched” highly reactive absorbent was prepared by mixing fly ash, lime and a small quantity of KMnO for simultaneous desulfurization and denitrification. Removal of SO and NO simultaneously was carried out using this absorbent in a flue gas circulating fluidized bed (CFB). The highest simultaneous removal efficiency, 94.5% of SO and 64.2% of NO, was achieved under the optimal experiment conditions. Scanning Electron Microscope (SEM) and Accessory X-ray Energy Spectrometer (EDX) were used to observe the surface characteristics of fly ash, lime, “Oxygen-enriched” highly reactive absorbent and the spent absorbent. An ion chromatograph (IC) and chemical analysis methods were used to determine the contents of sulfate, sulfite, nitrate and nitrite in the spent absorbents, the results showed that sulfate and nitrite were the main products for desulfurization and denitrification respectively. The mechanism of removing SO and NO simultaneously was proposed based on the analysis results of SEM, EDX, IC and the chemical analysis methods.

关键词： “Oxygen-enriched” highly reactive absorbent     Surface characteristics     Flue gas circulating fluidized bed     Simultaneous desulfurization and denitrification    

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标题 作者 时间 类型 操作

NADPH oxidase and reactive oxygen species as signaling molecules in carcinogenesis

Gang WANG

期刊论文

Reactive oxygen species generation is essential for cisplatin-induced accelerated senescence in hepatocellular

null

期刊论文

Resveratrol reduces intracellular reactive oxygen species levels by inducing autophagy through the AMPK-mTOR

Jun Song, Yeping Huang, Wenjian Zheng, Jing Yan, Min Cheng, Ruxing Zhao, Li Chen, Cheng Hu, Weiping Jia

期刊论文

concentrations of ionic silver promote the proliferation of human keratinocytes by inducing the production of reactiveoxygen species

null

期刊论文

Relationship between reactive oxygen species and sodium-selenite-induced DNA damage in HepG2 cells

ZOU Yunfeng, NIU Piye, GONG Zhiyong, YANG Jin, YUAN Jing, WU Tangchun, CHEN Xuemin

期刊论文

Association of gene variants with juvenile amyotrophic lateral sclerosis

期刊论文

Visible light induces bacteria to produce superoxide for manganese oxidation

期刊论文

INTERFERENCE BY NON-HOST PLANT ROOTS AND ROOT EXUDATES IN THE INFECTION PROCESSES OF PHYTOPHTHORA NICOTIANAE

期刊论文

Biotoxicity evaluation of zinc oxide nanoparticles on bacterial performance of activated sludge at COD, nitrogen, and phosphorus reduction

期刊论文

Discovery of novel ursolic acid derivatives as effective antimicrobial agents through a ROS-mediated apoptosis mechanism

期刊论文

Elimination of antibiotic resistance genes and control of horizontal transfer risk by UV-based treatment of drinking water: A mini review

Virender K. Sharma, Xin Yu, Thomas J. McDonald, Chetan Jinadatha, Dionysios D. Dionysiou, Mingbao Feng

期刊论文

Cooperative effect between copper species and oxygen vacancy in CeZrCuO catalysts for carbon monoxide

期刊论文

Review on design and evaluation of environmental photocatalysts

Xin Li, Jun Xie, Chuanjia Jiang, Jiaguo Yu, Pengyi Zhang

期刊论文

Light-induced variation in environmentally persistent free radicals and the generation of reactive radicalspecies in humic substances

Yafang Shi, Yunchao Dai, Ziwen Liu, Xiaofeng Nie, Song Zhao, Chi Zhang, Hanzhong Jia

期刊论文

Activity and characteristics of “Oxygen-enriched” highly reactive absorbent for simultaneous flue gas

Yi ZHAO,Tianxiang GUO,Zili ZANG

期刊论文